RT-qPCR validated differentially expressed miRNAs in EMT Hep3B exo and Hep3B exo. miR-125b-5p, hsa-miR-374a-5p, hsa-miR-24-3p, hsa-miR-200b-3p, and hsa-miR-21-5p were detected in the Hep3B exo, EMT-Hep3B exo, Hep3B cells, and Hep3B cells after treatment with EMT-Hep3B exo. *P < 0.05; **P < 0.01; ***P < 0.001.