Table 1.
Sequences of primers used for quantitative RT-PCR method
| Gene ID | Primer | Product Length |
|---|---|---|
| GAPDH | Forward primer | GAGTCAACGGATTTGGTCGT |
| Reverse primer | GACAAGCTTCCCGTTCTCAG | |
| GADD45A | Forward primer | GAAGGATCCTGCCTTAAGTCAAC |
| Reverse primer | CCTCTTTCCATCTGCAAAGTCATC | |
| HMOX1 | Forward primer | CTGGAGGAGGAGATTGAGCG |
| Reverse primer | TGGCACTGGCAATGTTGG | |
| E2F2 | Forward primer | TCCCAATCCCCTCCAGATC |
| Reverse primer | CAAGTTGTGCGATGCCTGC | |
| ARID3B | Forward primer | AGAGGACGGAGGTTTGGAAGA |
| Reverse primer | GCTGGAAGTAGATTGGACATGGG | |
| TUBB3 | Forward primer | GCAAGGTGCGTGAGGAGTAT |
| Reverse primer | GTCTGACACCTTGGGTGAGG | |
| U6 | Forward primer | CTCGCTTCGGCAGCACA |
| Reverse primer | AACGCTTCACGAATTTGCGT | |
| hsa-miR-125b-5p | RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCACAA |
| Forward primer | GCTCCCTGAGACCCTAACT | |
| hsa-miR-374a-5p | RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCACTTA |
| Forward primer | GGCGGTTATAATACAACCTGAT | |
| hsa-miR-24-3p | RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCTGTTC |
| Forward primer | ATGGCTCAGTTCAGCAGG | |
| hsa-miR-200b-3p | RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCATCA |
| Forward primer | GGCTAATACTGCCTGGTAATG | |
| hsa-miR-21-5p | RT | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCAACA |
| Forward primer | GCCGTAGCTTATCAGACTGA | |
| Universe-R | GTGCAGGGTCCGAGGT |