Table 2. Crystallization.

	Crystal used for SAD phasing	Crystal used for final structure refinement
Method	Sitting-drop vapour diffusion	Sitting-drop vapour diffusion
Plate type	Intelli-Plate 96-3	Intelli-Plate 96-3
Temperature (K)	293	293
Protein concentration (mg ml−1)	27	40
Buffer composition of protein solution	10 mM Tris–HCl pH 8.0, 150 mM NaCl	10 mM Tris–HCl pH 8.0, 150 mM NaCl
Composition of reservoir solution	15% glycerol, 8.5 mM NiCl2, 85 mM Tris–HCl pH 8.5, 17% PEG MME 2000	15.33% glycerol, 0.1 M MES–NaOH pH 6.3, 10.8% PEG 20 000
Volume and ratio of drop	200 nl:200 nl	200 nl:200 nl
Volume of reservoir (µl)	60	60