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. 2020 Apr 6;19:83. doi: 10.1186/s12934-020-01343-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — GFP only forms inclusion bodies during production on defined medium. SDS-PAGE analysis of IPTG induced cells carrying the gene encoding GFP growing on LB medium (lanes 1–6) and glucose supplemented mineral salt medium (lanes 8–13) collected 4 h after induction. Lanes 1, 10: N, T7⁺ RBS⁺ (non-induced controls, total cell extract of cells containing the expression vector with promoter and with RBS in front of the GFP gene), lanes 2, 11: W, T7⁺ RBS⁺ (induced cells with promoter and with RBS), lanes 3, 12: N, T7⁺ ΔRBS (non induced controls with promoter but without RBS), lanes 4, 13: W, T7⁺ ΔRBS (induced cells with promoter but without RBS), lanes 5, 8: S, T7⁺ RBS⁺ (soluble) and lanes 6, 9: I, T7⁺ RBS⁺ (insoluble cell fraction of induced cells containing the expression vector with promoter and with RBS). Lane 7: molecular mass marker, the position of GFP is indicated by an arrow. N: non-induced whole cell protein W: induced whole cell protein, S: induced soluble part, and I: induced insoluble part of whole cell protein