Fig. 7.

GFP transcript levels under inducing and non-inducing conditions. Transcript levels in E. coli BL21(DE3) transformed with expression vectors carrying the gene encoding GFP in shake-flask cultures with Luria–Bertani (LB) medium or defined glucose-supplemented mineral salt medium (DNB). Trancripts of genes encoding the major elongation factor EF-Tu (white bars) and GFP (gray bars) are shown in non-induced cells (open bars) and 4 h after IPTG induction (hatched bars). T7⁺ RBS⁺: cells containing the expression vector with promoter and with RBS in front of the GFP gene; T7⁺ ΔRBS: with promoter but without RBS. The insert depicts the same data set as the main figure only at different scale. Transcripts of rrsH were used as internal standard and artificially defined as 10,000. Data are presented as mean ± SD, n = 4