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. 2020 Apr 6;19:83. doi: 10.1186/s12934-020-01343-y

Table 1.

Plasmids

Plasmids Features
pET-28c/pET-29c Novagen, Germany
pET-28cΔRBS Mutant plasmid with deletion of all RBSs (AAGGAG) between T7 promoter and T7 terminator
pET-29c-hFGF-2 Parental plasmid for the production of hFGF-2 [15]
pET-29c-hFGF-2 ΔRBS Mutant plasmid with deletion of all RBSs (AAGGAG) between T7 promoter and T7 terminator
pET-29c-hFGF-2 ΔT7 Mutant plasmid with deletion of T7 promoter region (TAATACGACTCACTATAGG)
pET-29c-hFGF-2 ΔRBS & ΔT7 Mutant plasmid with deletion of all RBSs (AAGGAG) between T7 promoter and T7 terminator as well as T7 promoter region (TAATACGACTCACTATAGG)
pET-28c-His6-GFP Parental plasmid for the production of GFP [21]
pET-28c-His6-GFP ΔRBS Mutant plasmid with deletion of all RBSs (AAGGAG) between T7 promoter and T7 terminator