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Primers for qPCR
| Gene | Primer sequence 5′–3′ | Tm (°C) | Amplicon (bp) |
|---|---|---|---|
| rrsH | Fw: TCAGCGGGGATGAAGGGAGTAAAG | 58.0 | 209 |
| Rev: GCCAGTATCAGATGCAGTTCCCAG | 57.4 | ||
| tufA | Fw: TTCTTCAAAGGCTACCGTCCGC | 57.1 | 211 |
| Rev: GAACTTTAGCAACAACGCCCGC | 58.1 | ||
| hFGF-2 | Fw: AAGACCCGAAACGTCTGTACTGC | 57.1 | 215 |
| Rev: GGTAACGCATTTGGAAGCCAGC | 57.1 | ||
| GFP | Fw: TGGAAAACTACCTGTTCCCTGGC | 56.6 | 198 |
| Rev: CCTTCAAACTTGACTTCAGCACGC | 57.3 |
