Table 2.
Proteins with statistically significant altered abundance after surface shaving of S. haemolyticus incubated with human keratinocytes
| Accession | # PSM | # Unique Peptides | Fold change HaCaT vs Control | p-value HaCaT vs Control | Prediction of subcellular localization | Preferred name, EggNOG | Annotation summary |
|---|---|---|---|---|---|---|---|
| ACAKHAOO_01782 | 3 | 2 | 1.90 | 0.015 | Cytoplasmic | metK | S-adenosylmethionine synthase |
| ACAKHAOO_00208 | 8 | 7 | 1.75 | 0.046 | Extracellular (SPI) | ymaC | DUF867 type protein |
| ACAKHAOO_02015 | 7 | 2 | 1.60 | 0.014 | Extracellular (SPI) | sceD | Putative transglycosylase SceD |
| ACAKHAOO_02031 | 2 | 2 | 1.57 | 0.016 | Cytoplasmic | upp | Uracil phosphoribosyltransferase |
| ACAKHAOO_00454 | 6 | 3 | 1.55 | 0.027 | Cytoplasmic | ctc | 50S ribosomal protein L25 |
| ACAKHAOO_01033 | 54 | 25 | 1.46 | 0.039 | Extracellular (SPI) | atl | Bifunctional autolysin |
| ACAKHAOO_00250 | 4 | 3 | 1.40 | 0.044 | Cytoplasmic | – | TIR domain-containing protein |
| ACAKHAOO_00947 | 2 | 1 | 1.39 | 0.032 | Cytoplasmic | ppiB | Putative peptidyl-prolyl cis-trans isomerase |
| ACAKHAOO_02231 | 2 | 2 | 1.35 | 0.031 | Cytoplasmic | – | Putative oxidoreductase YghA |
| ACAKHAOO_01626 | 2 | 1 | 1.33 | 0.012 | Cytoplasmic | mnmA | tRNA-specific 2-thiouridylase MnmA |
| ACAKHAOO_01821 | 4 | 3 | 1.31 | 0.001 | Cytoplasmic | nagB | Glucosamine-6-phosphate deaminase |
| ACAKHAOO_00516 | 112 | 20 | 1.22 | 0.017 | Cytoplasmic | tuf | Elongation factor Tu |
| ACAKHAOO_00797 | 45 | 14 | −1.31 | 0.048 | Cytoplasmic | pgk | Phosphoglycerate kinase |
| ACAKHAOO_01712 | 7 | 5 | −1.44 | 0.026 | Cytoplasmic | ezrA | Septation ring formation regulator EzrA |
| ACAKHAOO_01065 | 2 | 1 | −1.51 | 0.004 | Cytoplasmic | – | DUF697 domain-containing protein |
| ACAKHAOO_02197 | 5 | 2 | −1.54 | 0.038 | Extracellular (SPI) | ssaA | Staphylococcal secretory antigen SsaA / CHAP domain-containing protein |
| ACAKHAOO_01875 | 14 | 5 | −1.65 | 0.034 | Cytoplasmic | yhbO | Uncharacterized protein SH1084 |
| ACAKHAOO_00904 | 9 | 4 | −1.74 | 0.026 | Surface (SPII)a | metQ | Methionine-binding lipoprotein MetQ |
| ACAKHAOO_01422 | 2 | 2 | −1.78 | 0.000 | Cytoplasmic | yaaN | TelA-like protein |
Surface proteins were defined as proteins predicted from cytoplasmic membrane, cell wall or extracellular origin. Positive prediction of subcellular localization was determined by a two out of three or greater concurrent results between the databases
a Surface: proteins were predicted as from cytoplasmic membrane, cell wall or extracellular origin, however, concurrent results between two out of three databases were not obtained