Fig. 6.

r-Aophytase enzyme kinetic parameters. a The released inorganic phosphorus gradually increased with an increase in the concentration of its substrate, phytic acid, when the enzyme amount kept constant. b Lineweaver–Burk plot and the corresponding fitting equation: y = 0.014 + 0.0138. Based on this equation, 72.46 μmol min⁻¹ mg⁻¹, K_m = 1.015 mM, and K_cat = 13.57 s⁻¹ were obtained