Table 1.
Methods and transcription factors for the generation of induced pluripotent stem cells
| Methods | Factors | Reference |
|---|---|---|
| Single lentiviral vectors expressing “stem cell cassette” with Cre-Lox transgene excision system | Oct4, Klf4, Sox2, cMyc | [24] |
| Cre-recombinase excisable DOX-inducible lentiviral vectors system | Oct4, Klf4, Sox2, cMyc | [25] |
| Repeated transfection of two expression plasmids without viral vectors system | (cDNA of Oct3/4, Sox2, Klf4) and cDNA of cMyc | [26] |
| Nonintegrating adenoviral vectors system | Oct4, Sox2, Klf4, cMyc | [27] |
| Nonintegrating Sendai virus-based vector system | Oct4, Sox2, Klf4, cMyc | [28] |
| Recombinant reprogramming proteins system (fusion of poly-arginine protein transduction domain to the C terminus of transcription factors) | Oct4, Sox2, Klf4, cMyc | [29] |
| Human artificial chromosomes (HACs) vectors (iHAC1 and iHAC2) system | Oct4, Sox2, Klf4, cMyc | [30] |
| Synthetic modified mRNA transfection system | Oct4, Sox2, Klf4, cMyc | [31] |
| Direct transfection of mature double-strand microRNAs (miRNAs) system | Combination of mir-200c, mir-302 s and mir-369 s | [32] |
| Doxycycline-inducible factors delivered by “piggyback” transposition system | Oct4, Sox2, Klf4, cMyc | [33] |
| Nonviral minicircle DNA vectors system | Oct4, Sox2, Nanog, Lin28 | [34] |
| Nonintegrating episomal plasmid vectors system | Oct4, Sox2, Nanog, Lin28, Klf4, cMyc | [35] |