Table 1. Macromolecule-production information.
Source organism | A. muciniphila (strain ATCC BAA-835/Muc) |
DNA source | Synthesized |
Forward primer | GATGAAGTTGATGCACATATGATTGTGAATAGCAAACGC |
Reverse primer | GTTAGCAGCCGGATCCTTCTCGAGTCATTAATCTTCGCTCGGTTCCTG |
Cloning vector | Modified pET-15b |
Expression vector | Modified pET-15b |
Expression host | E. coli BL21 (DE3) |
Complete amino-acid sequence of the construct produced | MGSSHHHHHHSSGLVPRGSHSDEVDAHMIVNSKRSELDKKISIAAKEIKSANAAEITPSRSSNEELEKELNRYAKAVGSLETAYKPFLASSALVPTTPTAFQNELKTFRDSLISSCKKKNILITDTSSWLGFQVYSTQAPSVQAASTLGFELKAINSLVNKLAECGLSKFIKVYRPQLPIETPANNPEESDEADQAPWTPMPLEIAFQGDRESVLKAMNAITGMQDYLFTVNSIRIRNERMMPPPIANPAAAKPAAAQPATGAASLTPADEAAAPAAPAIQQVIKPYMGKEQVFVQVSLNLVHFNQPKAQEPSED |