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. 2020 Apr 1;76(Pt 4):168–174. doi: 10.1107/S2053230X20004124

Table 1. Macromolecule-production information.

Source organism A. muciniphila (strain ATCC BAA-835/Muc)
DNA source Synthesized
Forward primer GATGAAGTTGATGCACATATGATTGTGAATAGCAAACGC
Reverse primer GTTAGCAGCCGGATCCTTCTCGAGTCATTAATCTTCGCTCGGTTCCTG
Cloning vector Modified pET-15b
Expression vector Modified pET-15b
Expression host E. coli BL21 (DE3)
Complete amino-acid sequence of the construct produced MGSSHHHHHHSSGLVPRGSHSDEVDAHMIVNSKRSELDKKISIAAKEIKSANAAEITPSRSSNEELEKELNRYAKAVGSLETAYKPFLASSALVPTTPTAFQNELKTFRDSLISSCKKKNILITDTSSWLGFQVYSTQAPSVQAASTLGFELKAINSLVNKLAECGLSKFIKVYRPQLPIETPANNPEESDEADQAPWTPMPLEIAFQGDRESVLKAMNAITGMQDYLFTVNSIRIRNERMMPPPIANPAAAKPAAAQPATGAASLTPADEAAAPAAPAIQQVIKPYMGKEQVFVQVSLNLVHFNQPKAQEPSED