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| Method | Vapor diffusion, sitting drop |
| Plate type | 48-well double-sample sitting-drop crystallization plate |
| Temperature (K) | 291 |
| Protein concentration (mg ml−1) | 20 |
| Buffer composition of protein solution | 25 mM Tris pH 8.0, 150 mM NaCl, 5 mM DTT |
| Composition of reservoir solution | 0.2 M K2HPO4, 1.8 M NaH2PO4, 0.1 M Na2HPO4/citric acid pH 4.2 |
| Volume and ratio of drop | 2 µl drop, 1:1 ratio |
| Volume of reservoir (µl) | 70 |
