Fig. 3.

Chronic alcohol feeding increases liver mRNA expression of TNFα and IL-6 and TLR4 coreceptors, CD14 and MD2, in WT, TLR2-deficient, and MyD88-deficient mice, but not in TLR4-KO mice. C57BL/6, TLR2-deficient, TLR4-deficient, or MyD88-deficient mice received the Lieber-DeCarli diet. Liver RNA levels of (A) TNFα, (B) IL-6, (C) CD14, and (D) MD2 were analyzed by real-time quantitative polymerase chain reaction (qPCR). The values were normalized to 18S and are shown as the fold increase over the pair-fed WT control group from N = 5–6 per group.