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. Author manuscript; available in PMC: 2020 Apr 7.
Published in final edited form as: Hepatology. 2008 Oct;48(4):1224–1231. doi: 10.1002/hep.22470

Fig. 4.

Fig. 4.

TLR4 deficiency protects from alcohol-induced increase of liver CYP2E1 expression. C57BL/6, TLR4-deficient, or MyD88-deficient mice (five to six per group) received the Lieber-DeCarli diet. (A) Liver RNA levels of CYP2E1 were analyzed by real-time quantitative polymerase chain reaction (qPCR). CYP2E1 protein level in liver microsomal fractions of (B) WT, (C) TLR4-deficient, and (D) MyD88-deficient mice was analyzed by western blot; calnexin expression was used as housekeeping control. A representative blot (top) and the densitometric analysis normalized to loading control from N = 3 (bottom) is shown.