Skip to main content
. Author manuscript; available in PMC: 2020 Apr 7.
Published in final edited form as: Hepatology. 2008 Oct;48(4):1224–1231. doi: 10.1002/hep.22470

Fig. 5.

Fig. 5.

Chronic alcohol feeding increases liver mRNA expression of the NADPH-complex subunits in WT, TLR2-deficient and MyD88-deficient mice but not in TLR4-KO mice. C57BL/6, TLR2-deficient, TLR4-deficient, or MyD88-deficient mice (five to six per group) received the Lieber-DeCarli diet. Liver RNA levels of (A) p22phox, (B) gp91phox, (C) p47phox, (D) p67phox, and (F) Nox4 were analyzed by real-time quantitative polymerase chain reaction (qPCR). The values were normalized to 18S and shown as fold increase over the pair-fed WT control group. (E) p47phox protein expression in whole liver membrane fractions was analyzed by western blot in alcohol-fed and pair-fed WT mice; pancadherin expression was used as a loading control. The densitometric analysis of p47phox protein expression (normalized to pan-cadherin; N = 3) is shown.