Fig. 2.

Knockdown of lncRNA CRNDE inhibits proliferation and metastasis in HCC cells. We divided the cells into four groups: pcDNA3, pcDNA3/lncRNA CRNDE, si-NC, and si-lncRNA CRNDE, transfected for 48 h, and then performed subsequent experiments. a MTT assay was performed to detect the proliferation ability of HCC cells when lncRNA CRNDE was overexpressed or knocked down. b FCM of apoptosis in HepG2 cells transfected with silncRNA CRNDE and siNC. c qRT-PCR and Western blot was used to detect the effects of lncRNA CRNDE on apoptosis associated genes. d, e Transwell migration and invasion assays were performed to detect the migration and invasion ability of QGY-7703 cells when lncRNA CRNDE was overexpressed or knocked down. f qRT-PCR and Western blot was used to detect the effects of lncRNA CRNDE on EMT associated genes. g, h Transwell migration and invasion assays were performed to detect the migration and invasion ability of HepG2 cells when lncRNA CRNDE was overexpressed or knocked down. i Tumor tissues in nude mice. *p < 0.05, **p < 0.01. All bars indicate the means±SD. All experiments were performed independently at least three times