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. 2020 Mar 31;11:531. doi: 10.3389/fimmu.2020.00531

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Copyright © 2020 Ali, Toews, Schwiebert, Klaus, Winkler, Grunewald, Oevermann, Deubzer, Tüns, Jensen, Henssen, Eggert, Schulte, Schwich, Rebmann, Schramm and Künkele.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of extracellular vesicles preparations derived from SH-SY5Y and SH-SY5Y-NTRK cells by Nanotracking analysis and immunoblotting. (A) The mean TEV size did not vary among the different TEV preparations (n = 9). (B) Immunoblotting of TEV preparation and cell lysates for typical TEV markers TSG101, Syntenin, and CD81. Calnexin as protein of endoplasmic reticulum served as negative control. (C) CD4⁺ and CD8⁺ T cell viability after 24 h co-culture with 10 μg extracellular vesicles, accessed via flow cytometry after live-dead staining. Depicted is mean of three independent experiments with each using technical triplicates with error bars representing standard deviation (SD).