Figure 2.

TEV-priming results in impaired CD4⁺ CAR T cell cytotoxicity. (A) Illustration of co-culture experiments with prior TEV-priming of CAR T cells. Co-culture experiments were conducted in three independent experiments with technical triplicates each. (B) Cytotoxicity assay of TEV-primed and unprimed CAR T cells measured biophotonically after co-incubation with GFP-ffluc expressing NTRK1 or NTRK2 expressing SH-SY5Y cell lines for 24 h at an effector:target ratio of 1:2. Co-culture experiments were conducted in three independent experiments with technical triplicates each. (C) Concentration of IL2 and (D) IFNG from supernatant of co-culture of TEV-primed and unprimed CAR T cells with tumor cells at an effector:target ratio of 1:2 quantified by ELISA. Cytokine measurements were conducted in three independent experiments with technical triplicates each. All experiments are depicted with error bars representing SD. *p < 0.05; **p < 0.01.