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. 2020 Mar 31;11:531. doi: 10.3389/fimmu.2020.00531

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Copyright © 2020 Ali, Toews, Schwiebert, Klaus, Winkler, Grunewald, Oevermann, Deubzer, Tüns, Jensen, Henssen, Eggert, Schulte, Schwich, Rebmann, Schramm and Künkele.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SH-SY5Y-NTRK1 increases CD171 expression in tumor cell lines and leads to improved CAR T cell cytotoxicity that is not significantly dictated through CD171 expression on the target tumor cell line. (A) Mean CD171 surface expression intensity (MFI) on SH-SY5Y-NTRK1, SH-SY5Y-NTRK2, and parental control by flow-cytometric analysis. Assessment of CD171 surface expression was done in three independent experiments. Expressed in mean with error bars representing SD. (B) Luciferase-based cytotoxicity assay of CD171-specific CAR T cells from CD4⁺ or CD8⁺ subsets against GFP-ffluc expressing SH-SY5Y-NTRK1, -NTRK2 and parental control measured after 24 and 72 h in an effector:target ratio of 2:1 and (C) 1:2. (D) Luciferase-based cytotoxicity assay of CD171-specific CAR T cells from CD4⁺ or CD8⁺ subsets against CD171-high SH-SY5Y-NTRK2 measured after 24 and 72 h in an effector:target ratio of 2:1 and (E) 1:2. Cytotoxicity assays were conducted in four independent experiments with technical triplicates each and expressed in mean with error bars representing SD. *p < 0.05; **p < 0.01.