Fig. 5.

Leucine-rich alpha-2 glycoprotein 1 (LRG1) could be induced by proinflammatory cytokines in HK-2 cell line and its effects on HUVEC cell line. a, b The expressions of LRG1 were analysed by western blot in HK-2 cells stimulated with 20 ng/mL IL-1β, IL-6, TNF-α and INF-γ for 8 h (n = 3). Analyses were performed by Student’s t test. c HUVEC cells stimulated by 500 ng/mL rhLRG1 for 8 h were double stained with FITC-conjugated anti-Annexin Vantibody and PI, followed by flowcytometry analysis for cell apoptosis. d CCK-8 assay was performed to assess cell proliferation in HUVEC cell line. e HUVEC cell line was stimulated by 500 ng/mL rhLRG1 for 24 h. The expression of mRNA was analyzed by real-time RT-PCR and GAPDH was used as an internal control for grayscale analysis. Data are shown as mean ± SEM. c-e analyses were controls groups vs. 500 ng/mL LRG1 stimulating groups performed by Student’s t test. n.s., not significant. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. LRG1: leucine-rich α2-glycoprotein 1; IL: Interleukin; TNF: Tumor necrosis factor; INF: Interferon; CCL: chemokine (C-C motif) ligand; CXCL: Chemokine (C-X-C Motif) Ligand 1