Fig. 2.

Ripasudil alleviated the inflammatory damage by mediating the expression of miR-136-5p. a The relative expression of miR-136-5p in RPE cells was detected with the RT-PCR. b The levels of miR-136-5p in RPE cells were determined after the application of miR-136-5p inhibitor. c The sequences of mature miR-136-5p and putative target sites of wild type ROCK1 and ROCK2. The mutant sequences of ROCK1 and ROCK2 were marked with red color. d Luciferase activity was determined in the miR-136-5p inhibitor and negative control groups. e The protein levels of ROCK1 and ROCK2 were detected after the application of miR-136-5p. *p < 0.05, **p < 0.01, ***p < 0.001