Figure 1.

Expression of JunB in Tregs and loss of immune homeostasis following Treg-restricted deletion of Junb. (A) Expression of JunB in Treg cells across tissues determined by scRNA-seq. (B) Histograms and (C) aggregate geometric mean fluorescent intensity (gMFI) of JunB protein expression in Treg cells from indicated tissues, representative data showing four mice from one of two independent experiments. (D) Body weights of Junb^F/+ (HET) or Junb^F/F (KO) Foxp3^YFPcre mice as determined at necropsy for 21 HET and 37 KO mice from 15 independent experiments. Statistical significance determined using Welch's t-test. (E) Frequencies of cytokine-producing cells among TCRβ⁺CD4⁺ cells in the indicated organs. Data are from at least six mice per group from four independent experiments. Significance determined by Mann-Whitney U-test with Holm-Bonferroni correction. (F) Representative contour plots and aggregate summary data for type 2 cytokines from at least six mice per group from four independent experiments, with pre-gating and statistics performed as in (E). All results represent mean with a bootstrapped 95% confidence interval. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant.