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. 2020 Mar 31;11:544. doi: 10.3389/fimmu.2020.00544

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Copyright © 2020 Wicker-Planquart, Dufour, Tacnet-Delorme, Bally, Delneste, Frachet, Housset and Thielens.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SPR analyses of the interaction of SR-F1 variants with C1q. SR-F1(20-421) (A), SR-F1(20-353) (B) and SR-F1(20-221) (C) were serially diluted and injected at five increasing concentrations in multiple cycle kinetics mode over covalently immobilized C1q (about 16,000 RU) in TBS-Ca-P at a flow rate of 20 μl/min. Fits (shown as red lines) and apparent K_D values were obtained by global fitting of the data using a 1:1 Langmuir binding model. (D) C1q, its collagen-like region (C1q-CLR) and its globular region (C1q-GR) were injected at the indicated concentrations over covalently immobilized SR-F1(20-421) (5,800 RU) in TBS-Ca-P at a flow rate of 20 μl/min. The data shown are representative of 3 (A–C) and 2 (D) separate experiments on different surfaces.