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. 2020 Mar 31;11:544. doi: 10.3389/fimmu.2020.00544

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Copyright © 2020 Wicker-Planquart, Dufour, Tacnet-Delorme, Bally, Delneste, Frachet, Housset and Thielens.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SR-F1 interacts with CRT and SR-F1 overexpression modulates the endocytosis of CRT. (A) CRT was serially diluted and injected at five increasing concentrations in single cycle kinetics mode over covalently immobilized SR-F1(20-421) (1,845 RU) in TBS-Ca-P at a flow rate of 30 μl/min. The fit (shown by a red line) and the apparent K_D value were obtained by global fitting of the data using a two-state reaction model. The curves shown are representative of two independent experiments. (B) Quantification of the endocytosis of soluble recombinant CRT (AF568-rCRT) by flow cytometry (MFI ratio ± SD, n = 3).