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. 2020 Mar 31;14:66. doi: 10.3389/fncel.2020.00066

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Copyright © 2020 von Ehr, Attaai, Neidert, Potru, Ruß, Zöller and Spittau.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of postnatal Mrc1 expression in microglia in vivo. (A) Workflow scheme depicting the time points for acute microglia isolation and flow cytometry. (B) Gating strategy and representative dot plots of F4/80⁺ and Mrc1⁺ microglia from P0, P14, and P28 brains. (C) Quantification of Mrc1⁺ microglia at the analyzed postnatal developmental stages. Data are given as percentages of F4/80⁺ microglia ± SEM [n = 12 (P0), n = 14 (P7), n = 10 (P14), n = 10 (P21), and n = 10 (P28)]. P-values derived from one-way ANOVA followed by Tukey’s multiple-comparison test are **p < 0.01 and ***p < 0.001.