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. 2020 Mar 31;11:299. doi: 10.3389/fphys.2020.00299

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Copyright © 2020 Zhao, Liu, Li, Chen, Shang, Yang, Jiang and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Femoral trabecular bone loss caused by 4 weeks of HLU in WT and Cx43 transgenic mice. μCT analysis reveals decreases in BV/TV (A) and Tb.N (B) and increases in SMI (C) and Tb.Sp (D); Tb.Th (E) is decreased in mutant but not in WT mice. Comparisons via two-way with Bonferroni test (A–E). Data represent the means ± SD. n = 6–10/group. *P < 0.05, **P < 0.01, ***P < 0.001. (F) μCT images of femoral trabecular microstructure. HLU, hindlimb unloading; WT, wild-type; μCT, micro-computed tomography; R, R76W; Δ, Δ130–136; BV/TV, bone volume fraction; Tb.N, trabecular number; SMI, structure model index; Tb.Sp, trabecular separation; Tb.Th, trabecular thickness.