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. 2020 Mar 31;11:299. doi: 10.3389/fphys.2020.00299

FIGURE 5.

FIGURE 5

HLU affects cortical osteocyte RANKL/OPG and sclerostin expression in Cx43 transgenic mice. (A,C) RANKL-positive osteocyte number is increased in Δ130–136 mice following HLU. (B,D) OPG-positive osteocytes number is increased only in WT mice. (E) RANKL/OPG-positive osteocyte number is increased in both R76W and Δ130–136 mice. (F,H) Sclerostin-positive osteocyte number is increased in the cortical bone of R76W and Δ130–136 mice. (G,I) TGF-β1-positive osteocyte number is increased in all three types of mice. Solid arrowheads indicate RANKL-, OPG-, sclerostin-, or TGF-β1-positive osteocytes. n = 3/group. Scale bar = 60 μm. Comparisons via two-way with Bonferroni test (C–E,H,I). Data represent the means ± SD. *P < 0.05, **P < 0.01. HLU, hindlimb unloading; WT, wild-type; R, R76W; Δ, Δ130–136.