Fig. 5.

Detection (GFP) of infectious virus progeny in the lysate of cBMDCe treated with LPS. (a) Schematic representation of experimental design. BMCs were isolated from BALB/c mice 3 months p.i. with RM4503 mutant MCMV, which expresses EGFP driven by HCMV MIEP, cultured for 9 days with GM-CSF/IL-4 containing medium, and pulsed with LPS for 48 h. Three days later, cell lysates made with treated with, or not treated with LPS were used to infect NIH3T3 cells. Four days p.i., the 3T3 cells were inspected under EVOS Digital Inverted Microscope for the expression of GFP. (b) Effect of LPS treatment on detection of GFP expression in LPS-treated cell lysates on NIH3T3 cells. Mock infection: 3T3 cells treated cell culture medium; LPS-: 3T3 cells treated with lysate of cBMDCe/LPS-; LPS+: 3T3 cells infected with lysate of cBMDCe/LPS+.