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. 2018 Nov 13;165(6):662–667. doi: 10.1099/mic.0.000739

Table 1. Transposon insertion mapping of the strains examined in this study.

Clone Mutated gene Enzyme tetM insertion point Phenotype
TNP1C23 MALL_0755 Extracellular sialidase nt 1530 of 1764 Extracellular NanI−
TNP1C556 MALL_0755 Extracellular sialidase In dcma Extracellular NanI−
TNP1C121 MALL_0641 Cytosolic sialidase nt 609 of 1536 Extracellular NanI+
TNP1C420 MALL_0641 Cytosolic sialidase nt 1209 of 1536 Extracellular NanI+
TP2C209 MALL_0639 N-acetylneuraminate lyase nt 11 of 885 Cytosolic NanA−
TP2C325 MALL_0639 N-acetylneuraminate lyase nt 596 of 885 Cytosolic NanA−

a, isogenic tetM insertion mapped to cytosine-5-methyltransferase gene dcm (MALL_0186) while extracellular sialidase-negative phenotype was conferred by spontaneous substitution mutations in MALL_0755.