Fig. 7.

Inhibition of periodontal pathogen sialidase activity by zanamivir. MUNANA was exposed to periodontal pathogens or purified sialidases. Sialidase activitywas expressed as the difference in 4-MU fluorescence relative to conditionswith no inhibitor. (a) MUNANA was exposed to T. forsythia or P. gingivalis in the presence of zanamivir for 1 and 4 hrs, respectively. Data represent themean of two experimental repeats, where each condition was performed three times per experiment. Error bars, sd. (b) MUNANA was exposed to SiaPG and NanHin the presence or absence of zanamivir (1-10 mM, 1 mM increments), and the IC50 of zanamivir for both enzymes obtained using the variable slope model in Graphpad Prism 7. Data represent the mean of three experimental repeats, where each condition was repeated three times. Error bars, sem.