FIGURE 6.

Vibrio vulnificus LPS and capsule profiles in response to temperature. R99 strain was grown until mid-log phase in CM9 at 20, 25, 28, and 37°C. Then, cell-associated polysaccharides (LPS + capsule) were extracted with the method of Hitchcock and Brown (1983) and quantified using the Total Carbohydrate Assay Kit (BioVision). Ten μg of cell associated-polysaccharides were separated by SDS-PAGE on discontinuous gels (4% stacking gel, 10% separating gel), transferred to a PVDF membrane, subjected to immunoblot analysis with antibodies against cell-envelopes of R99 according to Pajuelo et al. (2016). MMW, medium molecular weight.