TABLE 1.
Microarray validation by RT-qPCR.
| Comparisona | Gene | Fold changeb | |
| Array | RT-qPCR | ||
| 25°C vs. 20°C | ftbp | 2 (+) | 1.3 (=) |
| cpsA | 2 (+) | 4.9 (+) | |
| vvp | – | 1.5 (=) | |
| vvhA | – | 1.6 (=) | |
| rtxA13 | – | 1.9 (=) | |
| 28°C vs. 20°C | flp | 14 (++) | 3.6 (+) |
| malG | −3.1 (−) | −1.2 (=) | |
| vvp | – | 1.7 (=) | |
| vvhA | – | 1.3 (=) | |
| rtxA13 | – | 1.9 (=) | |
| 37°C vs. 20°C | vpsT | 5.1 (+) | 3.6 (+) |
| ktrA | 3.4 (+) | 1.6 (=) | |
| vvp | – | 1.9 (=) | |
| vvhA | – | 1.7 (=) | |
| rtxA13 | – | 1.4 (=) | |
Comparison of fold change values obtained by array and RT-qPCR using the V. vulnificus R99 strain. In case of RT-qPCR, results were obtained using recA as reference gene. The fold induction (2–ΔΔCt) for each gene was calculated. aRNA samples were obtained from mid-log phase cultures in CM9 at 20, 25, 28, and 37°C by using NucleoZOL (Macherey-Nagel), TURBOTM DNAse (Ambion) and GeneJET RNA Cleanup and Concentration Micro kit (Thermo Scientific). bQualitative classification of the fold change values: =, −2 ≤ X ≤ 2; +, 2 ≤ X ≤ 10; ++, 10 ≤ X ≤ 25; −, −10 ≤ X ≤ −2. –, not detected as differentially expressed by the microarray analysis.