Skip to main content
. 2020 Mar 31;12:78. doi: 10.3389/fnagi.2020.00078

FIGURE 3.

FIGURE 3

TBA attenuates 6-OHDA-induced cell death. (A) SH-SY5Y cells were incubated with TBA at indicated concentrations for 24 h, and cell viability was determined by CCK-8 assay. (B) Cells were pretreated with TBA for 2 h before exposure to 100 μM 6-OHDA for an additional 24 h. The effect of TBA on cell viability was measured by CCK-8 assay. (C) The effect of TBA on cell apoptosis was measured by Annexin-V/PI staining. The apoptotic rate calculated from the flow cytometry data was also shown. (D) The effects of TBA on apoptotic cells were determined by the TUNEL assay. (E) Real-time RT-PCR analysis of HDAC6 mRNA levels in siRNA-HDAC6-transfected cells. (F) Representative Western blotting and summarized data showing the relative HDAC6 expression in siRNA-HDAC6-transfected cells. (G) The effect of siRNA-HDAC6 on cell apoptosis was measured by Annexin-V/PI staining. Data are presented as mean ± SEM of three independent experiments. One-way ANOVA followed by Bonferroni post hoc test was used to assess significant differences among groups. *p < 0.05 vs. control; #p < 0.05 vs. the 6-OHDA-treated group.