Table 1.
Additional Axonal Transport-Related Measurements (A)
| Directional Movements | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Compound | Concentration | Anterograde | Retrograde | Stationary | Reversals | APP Particles | Pause Frequency | Pause Duration | ||||||||
| μM | % of All Particles | % of All Particles | % of All Particles | % of All Particles | # of MBOs/mm | # pauses/3min | sec | |||||||||
| Vehicle | 0.0 | 29.0± 4.57 | 18.4± 1.84 | 46.5± 4.00 | 6.1± 1.44 | 0.179± 0.0145 | 0.565± 0.0687 | 36.317± 2.609 | ||||||||
| DFP 1.0 nM Plus: | ||||||||||||||||
| Lithium Chloride | 0.0 | 22.5± 3.03 | 16.2± 2.02 | 57.5± 3.35 | 3.8± 0.975 | 0.186± 0.0128 | *0.946±0.0714 | 42.862± 2.454 | ||||||||
| 1.0 | 32.6± 3.69 | 13.0± 2.00 | 48.3± 3.59 | 6.1±1.39 | #0.224± 0.0138 | 1.199± 0.0696 | 41.882± 1.693 | |||||||||
| 10.0 | 32.2± 3.65 | 15.3± 2.19 | 47.1± 3.54 | 5.5±0.921 | 0.181± 0.0138 | 1.071± 0.0738 | 36.551± 1.736 | |||||||||
| 100.0 | 32.5± 2.87 | 17.8± 2.97 | 44.4± 2.94 | 5.3± 1.66 | 0.166± 0.0168 | 0.687± 0.0981 | 32.244± 2.577 | |||||||||
| 1000.0 | 33.3± 5.53 | 19.3± 4.15 | 39.8± 4.65 | 7.6± 2.47 | 0.179± 0.0224 | 0.931 ±0.0893 | 39.746± 2.848 | |||||||||
| 10000.0 | 24.4± 7.06 | 8.3± 3.02 | 59.4± 6.59 | 7.9± 2.19 | #0.112± 0.0158 | 0.975± 0.143 | 39.506± 4.593 | |||||||||
| Vehicle Plus Lithium Chloride | 100.0 | 34.5± 5.35 | 20.9± 3.91 | #35.8± 4.53 | 8.8± 1.58 | 0.175± 0.0171 | *1.182±0.0787 | 34.982 ± 1.465 | ||||||||
| Vehicle | 0.0 | 37.4 ± 2.93 | 14.3 ± 2.91 | 44.2 ± 2.82 | 4.1 ±0.825 | 0.219 ± 0.0187 | 0.679± 0.0685 | 40.010 ± 2.508 | ||||||||
| DFP 1.0 nM Plus: | ||||||||||||||||
| Methylene Blue | 0.0 | 26.3 ± 4.35 | 18.8 ± 3.45 | 49.5 ± 4.69 | 5.4 ±1.96 | 0.184 ± 0.0229 | 0.915± 0.0904 | 41.081 ± 2.926 | ||||||||
| 0.0001 | 21.9 ± 4.17 | 19.1 ± 2.38 | 48.9 ± 4.05 | 10.1 ±2.221 | 0.179 ± 0.0106 | #1.201± 0.0815 | 35.752 ± 1.695 | |||||||||
| 0.001 | 33.5 ± 5.00 | 18.9 ± 3.12 | 37.4 ± 4.23 | 10.3 ±1.99 | 0.203 ± 0.0181 | 0.884± 0.0787 | 34.541 ± 2.050 | |||||||||
| 0.01 | 42.0 ± 3.91 | 18.4 ± 2.52 | #33.2 ± 3.41 | 6.3 ±1.48 | 0.195 ± 0.0146 | 0.669± 0.0651 | 38.127 ± 2.336 | |||||||||
| 0.1 | 35.7 ± 5.02 | 17.0 ± 3.62 | 44.3 ± 3.80 | 3.0 ±0.874 | 0.192 ± 0.0165 | 0.759± 0.0810 | 40.406 ± 2.538 | |||||||||
| Vehicle Plus Methylene Blue | 0.01 | #44.7 ± 3.97 | 16.5 ± 309 | #30.8 ± 3.94 | 8.1 ±1.95 | 0.167 ± 0.0138 | 0.941± 0.0818 | 38.711 ± 2.368 | ||||||||
Each value represents the mean ± s.e.m obtained from 14–21 individual neurons from 3–5 separate experiments with 1–6 neurons per individual experiment.
*
= significantly (p<0.05) different from vehicle control
#
= significantly (p<0.05) different from 1nM DFP.