Figure 3.
Pathological examination and immunohistochemical staining from IBV variant‐infected chickens. One‐day‐old chicks were intranasally inoculated with 106 EID50 of IBV 2992/02. Tissues were collected from IBV‐ or mock‐infected chicks at 7 dpi. (a) Gross lesions from experimentally infected chicks showed congestion and haemorrhage in trachea and proventriculus, and urate deposit in kidney. (b) Immunohistochemical staining detected virus antigens (brown colour signals) in trachea, lung, proventriculus and kidney by IBV spike protein‐specific monoclonal antibody [Colour figure can be viewed at http://wileyonlinelibrary.com]