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. Author manuscript; available in PMC: 2020 Sep 25.
Published in final edited form as: Neuron. 2019 Jul 30;103(6):1016–1033.e10. doi: 10.1016/j.neuron.2019.07.002

Figure 7. xMGs down-regulate homeostatic markers and upregulate activation markers around Aβ plaques.

Figure 7.

Representative images of xMGs in the hippocampus and subiculum of the 5X-MITRG at 9-months. (A-H) The homeostatic marker P2RY12 is downregulated in plaque-associated xMGs while more distal xMGs continue to express P2RY12. (cytosolic GFP, green; P2RY12, pseudocolored red; fibrillar amyloid (AmyloGlo), gray). (I-P) Plaque-associated xMGs also display and increase in the DAM gene CD9, while distal cells do not (cytosolic GFP, green; CD9, red; fibrillar amyloid (AmyloGlo), gray). (Q-T) Additionally, plaque-associated xMGs upregulate other DAM markers, including MERTK (Q), human APOE (R), CD11C (S), and TREM2 (T). (U-X) 5X-MITRG mice were transplanted with xMGs possessing either WT TREM2 (U) or a homozygous R47H mutation (V) and aged to 9 months. Quantification of xMG migration towards Aβ plaques (blue, Amyglo) revealed a significant decrease in plaque-associated R47H-expressing xMGs (green, hNuclei/Ku80; red, IBA1), but no significant difference in total plaque burden (X). ** p<0.001, scale=500um (A-D), 100um (E-L), 20um (M-S; U-V), 10um (T). See also Video S3.