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. 2020 Mar 31;14:286. doi: 10.3389/fnins.2020.00286

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Copyright © 2020 Iba, Kim, Florio, Mante, Adame, Rockenstein, Kwon, Rissman and Masliah.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunoblot and RNA analysis for p38α and γ in DLB and control brains. Human frontal cortex samples were separated into cytosolic and membrane fractions by extracting sequentially with Tris and PDGF buffer and probed with p38α, p38γ, and β-actin. (A,B) Representative immunoblot images for (A) cytosolic and (B) membrane fractions probed with p38α, p38γ, and β-actin. In each panel, left six lanes show healthy controls and right eight lanes show DLB patients. (C,D) Image analysis of the cytosolic fraction immunoblot for p38α and p38γ, respectively. (E,F) Image analysis of the membrane fraction immunoblot for p38α and p38γ, respectively. (G,H) Real time qPCR for p38α and p38γ mRNA, respectively. n = 6 for control and n = 8 for DLB (*p < 0.05, **p < 0.01, ***p < 0.001).