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Cellular localization of PAF and PAFB and cell death induction in C. albicans in different media. Yeast cells were shaken at 200 rpm for 3 h at 30 °C in 0.1 × PDB or 0.1 × YPD in the presence of 8 μM BODIPY-labelled PAF and PAFB. Co-staining with 5 μg/mL propidium iodide was performed for 10 min before imaging. Images were taken with the same exposition time (1.500 ms). BF, Brightfield; BP, BODIPY-labelled proteins; PI, Propidium iodide.
