Table 4.

In silico predicted physicochemical properties of γ-core motives derived from PAF and PAFB and their anti-Candida efficacy.

Synthetic peptide	γ-core sequence§	Net charge at pH 7#	GRAVY$	IC90 [μM]⁎ C. albicans	References
Pγ		+1.8	−1.56	10	[14]
Pγopt		+4.8	−1.91	1.3	[14]
PBγ		+0.1	−0.72	>400	[28]
PBγopt		+4.8	−1.91	1.3	[28]

^§Peptides were synthesized with –SH group of the cysteines being reduced. Red letters indicate aa exchanges.

^#Protein Calculator v3.4 (The Scripps Research Institute; http://protcalc.sourceforge.net/) was applied for net charge calculation.

^$GRAVY: Grand Average of Hydropathy.

^⁎IC₉₀, AMP concentration inhibiting growth ≥90%.