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. 2020 Mar 31;11(13):1097–1108. doi: 10.18632/oncotarget.27425

Figure 4. Fli-1, a transcription factor in the upstream region of HSPB1.

Figure 4

(A) Quantification of ChIP DNA by standard PCR of nine predicted binding sites in chromatin from SVGP12 and T98G cells. (B) Quantification of ChIP DNA by standard PCR of selected binding sites (primer no. 3, 6 and 7) in chromatin from human adjacent normal and GBM tissues. (C) Quantitative Real-Time PCR of selected binding sites (primer no. 3, 6 and 7) in SVGP12 and T98G cells; human adjacent normal and GBM tissue. (D) RT-PCR image showing no amplification of ChIP DNA after mutation of binding sites 3, 6 and 7 with their respective controls.