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. 2020 Mar 31;11(13):1097–1108. doi: 10.18632/oncotarget.27425

Figure 5. Fli-1 overexpressing stable U87MG cells displaying radio/TMZ resistance-like characteristics.

Figure 5

(A, B) Determination of survival fraction and IC50 of radiation and TMZ in parental U87MG cells, radiation-resistant U87MG RR, TMZ-resistant U87MG and U87MG Fli-1 cells. (C) Western blotting analysis of Fli-1, HSPB1, MMP-2, MMP-9 and EMT proteins in U87MG, U87MG RR, U87MG TMZR and U87MG Fli-1 cells. Densitometry of (C) is in Supplementary Figure 6. During contrast adjustments of certain blots in panel c, background removal occurred. (D) Phase contrast microscopy images of U87MG RR/TMZR and U87MG Fli-1 cells. U87MG Fli-1 cells showing long cellular extensions (EMT-like traits) comparable to resistant cells. (E) Soft-Agar assays evaluating anchorage independent growth of U87MG, U87MG RR, U87MG TMZR, U87MG Fli-1 cells. (F) Wound-healing assays evaluating migratory potential of U87MG, U87MG RR, U87MG TMZR, U87MG Fli-1 cells.