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. 2020 Mar 31;11(13):1097–1108. doi: 10.18632/oncotarget.27425

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Copyright: Rajesh et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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(A, B) Determination of survival fraction and IC₅₀ of radiation and TMZ in parental T98G, radiation-resistant T98G RR, TMZ-resistant T98G TMZR, T98G RR Fli-1 shRNA and T98G TMZR Fli-1 shRNA cells. (C) Phase contrast microscopy images of parental T98G, radiation-resistant T98G RR, TMZ-resistant T98G TMZR, T98G RR Fli-1 shRNA and T98G TMZR Fli-1 shRNA cells. T98G RR Fli-1 shRNA and T98G TMZR Fli-1 shRNA cells showing absence of long cellular extensions (EMT-like traits) similar to T98G cells. (D) Wound-healing assay evaluating migratory potential of parental T98G, radiation-resistant T98G RR, TMZ-resistant T98G TMZR, T98G RR Fli-1 shRNA and T98G TMZR Fli-1 shRNA cells. (E, F) Western blotting analysis of Fli-1, HSPB1, MMP-2, MMP-9 and EMT proteins in parental T98G, radiation-resistant T98G RR, TMZ-resistant T98G TMZR, T98G RR Fli-1 shRNA and T98G TMZR Fli-1 shRNA cells. Densitometry plot of (E, F) is in Supplementary Figure 7A and 7B. During contrast adjustments of certain blots in panels e and f, background removal occurred.