Figure 1.

(A) miR-155 expression in clinical samples was quantified by q-PCR and normalized with respect to U6 as housekeeping gene. miR-155 expression profiling in cis^Res and cis^Sens oral cancer cells at the (B) cellular and (C) exosomal level. (D and E) Western blot analysis for the exosomal marker CD-9 in exosomes isolated from serum of clinical samples and cis^Res and cis^Sens SCC-131 oral cancer cells. In the absence of a well-accepted standard internal control, densitometric analysis was done by normalizing the CD9 blot with a prominent band visualized on the PVDF membrane following Ponceau-S staining. Data are expressed as mean +/– SD.^* p < 0.05 and ^** p < 0.01 compared with the cis^Sens cells or healthy controls in case of clinical samples. (n = 3). Two independent experiments gave similar results.