Figure 2.

(A) Binding position prediction of miR-155 with FOXO3 using TargetScan, DIANA microT-CDS and rna22 web-based tools. (B) FOXO3a luciferase activity in cis^Sens cells co-transfected with either NTC or miR-155 mimics and the cloned p-mirGLO-FOXO3a dual luciferase vector. Data are expressed as the mean +/– SD. ^** p < 0.01, significant difference vs. NTC group (n = 3). Two independent experiments gave similar results. Following transfection of miR-155 mimics in cis^Sens cells, miR-155 expression was validated by q-PCR at both the (C) cellular and (D) exosomal level. FOXO3a expression was measured by (E) q-PCR and (F) Western Blot. (G) Densitometry analysis of FOXO3a western blot normalized to β-actin as the loading control. Data are expressed as the mean +/– SD. ^* p < 0.05 and ^** p < 0.01. (n = 3). Two independent experiments gave similar results.