Figure 4. Exosomes were isolated from cis^Sens and cis^Res cells transfected with either NTC or miR-155 mimics, and were used to treat naïve cis^Sens cells.

cis^Sens cells were employed as controls and for comparison. (A) miR-155 were quantified by q-PCR and normalized with respect to U6 as housekeeping gene and (B) FOXO3a expression was quantified by q-PCR and normalized with respect to 18S as housekeeping gene. (C) FOXO3a expression was measured by Western Blot. Densitometry analysis of FOXO3a western blot normalized to β-actin as the loading control. (D) Following exosomes conditioning treatment, cells were exposed to different concentrations of cisplatin (μM, as indicated) for 48 h. Cell viability was measured by the MTT assay. Data are expressed as the mean +/– SD. ^* p < 0.05 and ^** p < 0.01 (n = 3). Two independent experiments gave similar results. (E) After cisplatin treatment, cells were harvested, stained with PI and analysed for cell distribution into different phases of cell cycle using FACS S3e cell sorter (Bio-Rad).