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. 2020 Mar 31;11:462. doi: 10.3389/fimmu.2020.00462

Figure 4.

Figure 4

Contributory role of classical and alternative pathways in C3b deposition on Bacillus anthracis. Flow cytometry histogram for C3b deposition on non-encapsulated (A) and encapsulated (B) B. anthracis strains incubated in 10% human serum deficient in complement component C1q (C1q–), factor D (FD–), and C5 (C5–). Gray, red, and blue histogram shading represents C3b deposition in C1q–, C5–, and FD– deficient sera, respectively. (C) C3b binding represented as mean fluorescence index (MFI) observed with non-encapsulated (black bars) and encapsulated (gray bars) B. anthracis strains incubated in C1q and FD sera. Each bar represents the mean of three independent experiments. Error bars represent standard error of the mean. Statistical significance is highlighted by the following denotations: *** for P-value < 0.001.