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. 2020 Mar 31;11:462. doi: 10.3389/fimmu.2020.00462

Figure 5.

Figure 5

Binding of complement mediators with encapsulated and non-encapsulated strains of Bacillus anthracis. Flow cytometry histogram for binding of pentraxins C-reactive protein (CRP) (A) and serum amyloid P component (SAP) (B) on B. anthracis strains incubated in 10% human serum. Gray and red shading indicates non-encapsulated and encapsulated bacteria, respectively. (C) CRP and SAP binding represented as mean fluorescence index (MFI) observed with non-encapsulated (black bars) and encapsulated bacteria (gray bars). Each bar represents the mean of three independent experiments. Error bars represent standard error of the mean. The scatter plots for the corresponding histograms are represented in Figures S4, S5. (D,E) Immunoblot assay for detection of human serum IgG and purified human IgG binding with B. anthracis non-encapsulated strains (D) and encapsulated strains (E) incubated with increasing concentration of normal human serum (5–50%) and purified human IgG (1–100 μg/ml); 1% normal human serum and 10 μg/ml of IgG was used as positive control. Statistical significance is highlighted by the following denotations: *** for P-value < 0.001.