Table 1.
Compound | λabs (nm)a |
λem (nm)b |
ε (M−1cm−1) |
Φflc | Brightness (M−1cm−1) |
---|---|---|---|---|---|
NO2-Rosol (unactivated form) |
550 | 710 | 11,000 | 0.0005 | 5.5 |
NH2-Rosol (activated form) |
548 | 705 | 10,300 | 0.0205 | 210 |
Measured maximum absorption wavelength of each compound in buffer;
Measured maximum fluorescence emission wavelength of each compound upon photoexcitation at its respective λabs in buffer.
Φfl is the measured relative fluorescence quanum yield using erythrosine B in buffer as a reference (Φfl = 0.02).