Figure 2.

The homology model of the RETREG1 RHD (amino acid residues 80–240) predicted by Phyre2 based on the amino acid sequence obtained in the UniProt database. The α-helical feature was modeled by Phyre2 based on the Nogo-66 NMR structure (PDB ID: 2KO2). The rest of the model, including the TM segments, is a low confidence prediction. Modeling to the best of the ability of the fold recognition server cannot reveal the functional topology of RETREG1 without a direct input from lipid membranes.