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. Author manuscript; available in PMC: 2021 Jan 27.
Published in final edited form as: Dev Cell. 2020 Jan 27;52(2):141–151.e5. doi: 10.1016/j.devcel.2019.12.017

Figure 4: Autophagy inhibition affects diverse cell cycle regulators.

Figure 4:

A,B) Representative time-lapse images of an atg1-as cell undergoing synchronized meiosis in the absence (A) and presence (B) of 1-NM-PP1. Blue arrows show Cdc14 release from the nucleolus. Scale Bar: 5μm. C) Graph showing the percent of cells in part B with the indicated number of Cdc14 releases after meiosis II (n=50 cells). D,E) Western blotting analysis of the indicated cell cycle regulators following release from prophase I arrest in the absence (D) and presence of 1-NM-PP1 (E). Nop1 is a nucleolar protein used as a loading control. F,G) Representative time-lapse images of atg1-as cells undergoing synchronized meiosis in the absence (A) and presence of 1-NM-PP1 (B). Scale Bars: 5μm.